New attL fragment is actually PCR increased of pCMVssattL using primers attL(BOP?)Fwd-(KpnI) and you can attL(BOP?)Rev-(NotI)

New attL fragment is actually PCR increased of pCMVssattL using primers attL(BOP?)Fwd-(KpnI) and you can attL(BOP?)Rev-(NotI) pTZ-attP4X-UN-EF1?-eGFP try generated by subcloning the brand new attP4X succession out-of pattP4X-PGKssPuro while the a keen EcoRI fragment from the unique EcoRI website upstream of the individual UTF1 promoter in pTZ-United nations ( dos5) to generate pTZ-attP4X-Us. The EF1?-eGFP

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